Investigation of Eucalyptus and nanosilver as a new nanomixture for growth inhibition of E.coli

Document Type: Research Article


1 Department of Biology, Falavarjan branch, Islamic Azad University, Isfahan, Iran.

2 Department of Microbiology, Falavarjan branch, Islamic Azad University, Isfahan, Iran.

3 Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.

4 Department of Biochemistry, Falavarjan branch, Islamic Azad University, Isfahan, Iran.


Nanotechnology is a new and specific method for therapy of diseases. Nanosilver particle is one of the functional fields of nanotechnology. It has been shown that these nanoparticles have antibacterial and antifungal properties. Different studied have been shown that nanoparticles may have important role in medicine. For example, it has been shown to have inhibitory effects on the treatment of many diseases. The shape and size of nanoparticles are very important. In this study the inhibitory effects of silver on E. coli has been investigated. Nanosilver in different concentrations on inseminated blank antibiogram disk was placed on cultivated nutrient agar environment by 0.5 Mac Farland`s standard. After the first, second, and eight day the diameter of disks was measured. It was found that nanosilver in a concentration of 50ppm is more effective than the other concentrations for growth inhibition of E. coli. The best time for induction of inhibitory effects on E.coli was 3 day post treatment with silver nanoparticles. Investigation of inhibitory effects for nanosilver concentration on E. coli is an example of functional effects of new nanobiotechnlogy. It was shown that eucalyptus and nanosilver has the maximum inhibitory effects on the growth of E.coli 1 day post treatment. The results from this study suggest that silver nanoparticles in combination with eucalyptus extracts may be useful for the therapy of human bacterial diseases.