• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Indexing and Abstracting
    • Related Links
    • FAQ
    • Peer Review Process
    • News
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter
International Journal of Molecular and Clinical Microbiology
arrow Articles in Press
arrow Current Issue
Journal Archive
Volume Volume 9 (2019)
Volume Volume 8 (2018)
Volume Volume 7 (2017)
Volume Volume 6 (2016)
Issue Issue 2
Issue Issue 1
Volume Volume 5 (2015)
Volume Volume 4 (2014)
Volume Volume 3 (2013)
Volume Volume 2 (2012)
Volume Volume 1 (2011)
Tahamtan, Y., Sahragard, I. (2016). Characterization, ribotyping and capsular PCR typing of Pasteurella multocida isolated from Iranian sheep and goats. International Journal of Molecular and Clinical Microbiology, 6(1), 602-607.
Yahya Tahamtan; Ilnaz Sahragard. "Characterization, ribotyping and capsular PCR typing of Pasteurella multocida isolated from Iranian sheep and goats". International Journal of Molecular and Clinical Microbiology, 6, 1, 2016, 602-607.
Tahamtan, Y., Sahragard, I. (2016). 'Characterization, ribotyping and capsular PCR typing of Pasteurella multocida isolated from Iranian sheep and goats', International Journal of Molecular and Clinical Microbiology, 6(1), pp. 602-607.
Tahamtan, Y., Sahragard, I. Characterization, ribotyping and capsular PCR typing of Pasteurella multocida isolated from Iranian sheep and goats. International Journal of Molecular and Clinical Microbiology, 2016; 6(1): 602-607.

Characterization, ribotyping and capsular PCR typing of Pasteurella multocida isolated from Iranian sheep and goats

Article 2, Volume 6, Issue 1, Summer and Autumn 2016, Page 602-607  XML PDF (221.96 K)
Document Type: Research Article
Authors
Yahya Tahamtan email 1; Ilnaz Sahragard2
1Sanaye
2Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
Abstract
Pasteurella multocida is considered to be an important cause of ovine pneumonia and results causes considerable economic losses in Iran. Ribotyping PCR (Ribo-PCR) was used for investigating the diversity of ovine and caprine P.multocida. A total of 120 swab tonsil and nasal samples were obtained from sheep and goats. They were analyzed by biochemical tests and Ribo-PCR 16S-23S ribosomal RNA genes. Twenty samples representing Pasteurella phenotypes by Entero rapid kit and 17 out of 20 isolates were identified as P.multocida. Capsular type A was dominant among the isolates and was variable than serogroup D. Nine isolates including JF694004.1 and 8 isolates including JF681973.1 showed 100% and 94% similarity, respectively. Two minor cluster I and II was obtained with no significant diversity among the isolates. Cluster II was divided in four sub cluster including IIa, IIb, IIc and IId. Molecular approaches such as Ribo-PCR are necessary for determining and characterizing the diversity of P. multocida and understanding their interactions with host. The study has been the sensitive levels of Ribo-PCR in epidemiological studies of pasteurellosis, however, correlation among diversity of isolates and host origin is not fully understood.
Keywords
PCR-Ribotype; Pasteurella multocida; Sheep; goats; Iran
Main Subjects
Molecular Microbiology
Statistics
Article View: 495
PDF Download: 462
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by sinaweb.