A study on Antimicrobial Resistance of Helicobacter Pylori Isolates Recovered from Dyspeptic Patients by Epsilometer Test

Document Type: Research Article

Authors

Department of Biotechnology, Lahijan branch, Islamic Azad University of Iran

Abstract

The main purpose of the study is to determine resistance pattern of bacterial isolates to selected antibiotics among 88 dyspeptic patients in Iran. Frequency of infection among these patients have been evaluated among dyspeptic patients old (45 boys and 43 girls, aged 5-18 years) from 2016 to 2017.Eighty-eight patients with gastroduodenal disorders were included in the study. Urease test modified Giemsa stain, culture isolation and histopathology of H.pylori isolates were performed as per standard guidelines. H.pylori ATCC 43504 was used as reference strain. Susceptibility of HP isolates to amoxicillin, tetracycline, metronidazole, clarithromycin and levofloxacin was determined using EUCAST break points by E-test method. Antral gastritis caused by H pylori infection (15.91%) with or without extension into the duodenum was prominent endoscopic finding. The study revealed modified Giemsa stain positivity of 13.6%, urease test positivity 23.9%, and culture positivity rate of 18.2%. The three tests Culture, Giemsa, and Urease test found to be significant statistically (P<0.05). H. pylori isolates indicated the most sensitivity against tetracycline, amoxicillin, and levofloxacin. The most rates of resistance of HP isolates were shown to Clarithromycin and metronidazole. Findings showed that the MICs of therapeutic antibiotics for eradication of HP have increased in the past 10 years in northern Iran. Culture and histopathology, urease test, modified Giemsa smear and antibiogram results proved to be helpful in the diagnosis of H. pylori infection. Antimicrobial susceptibility testing can play an important role in experimental treatment of HP infection and management of drug resistant cases of isolates in our country.

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