Document Type: Research Article
Islamic Azad University, Fars Science Research Branch, Shiraz, Iran
Islamic Azad University, Tonekabon Branch, young researchers club, Tonekabon, Iran
Islamic Azad University, Kazeroun Branch, Kazeroun, Iran
The major purpose of this study was isolation, identification and antimicrobial susceptibility of thermophilic Campylobacter spp. from different sources including domestic animals (cow, sheep, horses), poultry, river water and sewage in Tonekabon. Campylobacter spp. was isolated using prêt-KB method and identified by phenotyping tests. Antimicrobial susceptibility of the isolates against different antibiotics and Minimal Inhibitory Concentration (MIC) values were determined by disc diffusion and double dilution methods respectively. In general, 32 strains of thermophilic Campylobacter were isolated from all of the sources. The results obtained indicated that frequency of occurrence of Campylobacter in poultry was high and in sewage was low. In addition, thermophilic Campylobacter isolates were sensitive to Amikacin and Ciprofloxacin and resistant to Ampicillin, Amoxicillin, Penicillin, Amoxiclave and Vancomycin. The lowest values of MIC were found for Ciprofloxacin, while the highest value was found for Streptomycin. Overall, our observations, illustrated that pathogenic Campylobacter were existed in all of the sources in north of Iran. Furthermore, they were sensitive to Amikacin and Ciprofloxacin and resistant to β lactam antibiotics.