Isolation and identification of bioactive compound producing Rhodococcus spp. isolated from soil samples

Document Type: Research Article

Authors

1 Department of Microbiology, Science and Research Branch, Islamic Azad University, Fars, Iran; Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran

2 Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran

3 Department of Microbiology, Kazeroun Branch, Islamic Azad University, Kazeroun, Iran

Abstract

The genus Rhodococcus is gram positive bacterium which was classified into the family of Nocardiaceae.  The organism can be isolates from different sources, with capability to degrade diverse types of pollutant. Nowadays, scientists reported a few secondary metabolites which are produced by Rhodococci. Hence, the present study tried to isolate Rhodococci from different soil samples with the capability to produce antimicrobial metabolites. For this purpose 360 soil samples were collected from different types of soil. Then the isolates were identified and characterized using APi Coryne kit. Afterward antibacterial activity of the suspected bacteria was evaluated using well diffusion agar method against test bacteria. Furthermore, the extracted metabolites were analysed using GC-MS. The results obtained from this study indicated that 4 out of 9 isolates were able to produce antimicrobial metabolites. The most effective isolates were R87 strain with the maximum inhibition zone of 35 mm and the least effective were R85 strain with zone of inhibition 20 mm against E. coli. The GC-Mass chromatogram showed different special peaks and the identified compounds were cyclopenthasiloxane, Azulene, 2-7 octadiene, docosane, cyclohexasiloxane, Bis (3,5,5 trimethyhexyl, Tetraedacane – 2 methyl, Cycloheptasiloxane  and Heptadecanol. In general, isolation of native bacteria from environmental samples with antimicrobial property is an interesting aspect which could help us to ignore antibiotic resistant bacteria from our environment.  

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