Document Type: Research Article
Department of microbiology, Science and research branch, Islamic Azad University, save, Iran
Department of Medical Mycology and parasitology,school of Medicine,Iran university of medical science,tehran,iran
Candida albicans has the ability to change between yeast and hyphal cells and is known to be a virulence property. Efg1gene of C.albicans is as a main transcription factor that plays pivotal roles in biofilm formation The aim of the current study is to investigate the presence of Efg1 gene in Candida albicans isolates from women with vaginal candidiasis and its impact on biofilm formation.
We used 50clinical isolates subjected to PCR-RFLP to confirm C.albicans. Total RNA was extracted from C. albicans isolates by glass bead and lysis buffer, and cDNA was synthesized using Reverse Transcriptase enzyme. RT-PCR was used to evaluate the expression of Efg1 gene. Biofilm formation was evaluated in 96-well microplate and then tetrazolium reduction was assayed. All data were analyzed using t-test .
50 clinical isolates out of 150 were confirmed as C.albicans by using PCR-RFLP. 47(94%) isolates out of 50 present Efg1 gene by using PCR, and 12(25.53%) isolates out of 47 expressed Efg1 gene by the RT-PCR. Isolates which have Efg1gene successfully form dense biofilm on microplate. The results of the tetrazolium reduction assay test showed that 2 isolates with Efg1 gene expression and 2 isolates lacked Efg1 gene expression which was significant at P-value of0.014 (P<0.05).
Results of MTT assay identified biofilm formation in isolates with Efg1gene expression and no biofilm formation in isolates which no Efg1 gene expression.It could then be concluded that the growth of hyphae is influenced by environmental factors which is also essential for biofilm formation.