Document Type: Research Article
Department of Microbiology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
Department of Microbiology, Kazeroun Branch, Islamic Azad University, Kazeroun, Iran
Department of Burn and Wound Healing Research Center, Shiraz University of medical sciences, Shiraz, Iran
Although, many genes are involved in pathogenesis of Campylobacter, racR gene considered the main gene for Campylobacter pathogenicity in humans. The purpose of this study was determined the prevalence of recR gene in Campylobacter spp. isolated from domestic animals and water.
To perform the study 392 fecal and water samples were collected from poultry(182), cow(141), sheep and goat(41) and water(28). All samples were subjected for isolation of Campylobacter spp. using prêt KB method and the presumptive isolates authenticated by DNA sequencing of 16srRNA genes. Finally, Campylobacter isolates assessed for detection of racR gene. The results obtained indicated that 50 strains of Campylobacter spp. were isolated. High isolate frequency (37/50) was for Poultry and low frequency (2/50) was for sheep and goat. Of All isolates thirty six strains were identified as Campylobacter jejuni and the rest (14 isolates) was Campylobacter coli. The genome of 30(83.3%) C.jejuni and 14(100%) C.coli were contained racR gene. Hence based on foregoing evidence racR gene existed approximately in all isolates. Therefore, Campylobacter strains isolated from different sources could be considered infectious agent for human. It is because phenotypical character inducted by racR gene (colonization and heat tolerance) helps them to survive and cause infection.