Evaluation of Mycoplasma Muris and Pulmonis infections in Genital and Respiratory Tracts

Document Type: Research Article

Authors

1 Department of Laboratory Animal Breeding, Agricultural Research, Education and Extension Organization, Razi Vaccine and Serum Research Institute, PO Box 31975/148 Hessarak, Karaj, Alborz, Iran

2 MSc in Genetics, Dept. of Biology, Tonekabon Branch, Islamic Azad University, Mazandaran, Iran

3 Mycoplasma reference laboratory, Razi vaccine and serum research institute, Karaj, Iran

4 Faculty of Biological Science, Islamic Azad University, Tonekabon Branch, Mazanderan, Tonekabon, Iran

5 Quality Assurance, Razi Vaccine and Serum Research Institute, Karaj, Iran

6 Research and Development Department, Agricultural Research, Education and Extension Organization, Razi Vaccine and Serum Research Institute,

7 Department of Research & Development, Razi Vaccine and Serum Research Institute, Karaj, Iran

Abstract

Abstract
Mycoplasma pulmonis and muris infections have been associated with several diseases in conventionally housed laboratory rat and mice colonies. In naturally infected of mycoplasma pulmonis in mice and rat colonies, the respiratory organ appears to be the favored site of colonization; so, it was not surprising to see that infection spread from the site of urogenital tract to the respiratory tract. In this research, PPLO broth culture and polymerase chain reaction (PCR) assay was used to detect Mycoplasma pulmonis and muris contamination in mice strain NIH. A new species of Mycoplasma muris(MYMORazi) have been identified in the nasopharyngeal, lung and vaginal samples of mice strain NIH in Razi Vaccine and serum research institute. Despite using two pair's specific primers targeting 16SrRNA gene of Mycoplasma pulmonis, detection was failed in both organs. We propose that the Mycoplasma muris strain MYMO Razi can cause infection in both organs of mouse strain NIH without presence of Mycoplasma pulmonis.

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