Comparison of Culture and Molecular Techniques for Identifying Gardenerlla vaginalis in Urinary Tract Infection

Document Type: Research Article

Authors

Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, IRAN.

Abstract

Urinary tract infection is the most prevalent infection in the human societies. This study aims to identify G. vaginalis in the urinary samples of the patients suffered from UTI by culture and PCR techniques. 200 patients with urinary tract infection signs have entered in this study. urine sample was cultured in the blood agar medium, a small quantity of the sample used for the purpose of molecular technique. In order to analyze the data, SPSS software and chi square (x2) test has been used. Of 200 studied patients, G. vaginalis was isolated and identified in 6 (3%) and 29 (14.5%) patients with the culture and PCR techniques, respectively. The sequencing of the samples confirmed phenotyping identification. On the basis of the results obtained from both two techniques, it can be expressed that PCR technique is more appropriate to identify G. vaginalis. The achieved results show that G. vaginalis is regarded as one the agents causing the occurrence of urine infection. Yet, in many medical diagnosis laboratories, the bacterium is undetectable due to hardly growth. Also, considering the conducted techniques, PCR method is of a higher accuracy and speed.

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