Anti-Helicobacter pylori Potential of Podophyllotoxin: In Silico Study

Document Type : Research Article


Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran


Helicobacter pylori is probably the most common chronic bacterial infection in humans and has infected almost half of the world's population. Podophyllotoxin is a lignan compound of plant origin and has great medicinal importance due to its various biological properties. The aim of this study was to investigate the podophyllotoxin as Urease, VacA and cagA inhibitor of Helicobacter pylori. The crystallized structure of podophyllotoxin was received from the Zinc database and used as a ligand. The structure of the ligand was optimized by the mm2 method with Chem3D v20.1.1.125 software. The ligand was evaluated as an inhibitor against the active site of the urease enzyme, VacA, and cagA by AutodackVina software. The output results were analyzed and evaluated by Discovery Studio v16.1.0 software. The best affinity was obtained against VacA = -8.1 kcal/mol. The highest diversity of links was also reported in VacA. Hydrogen bonds established with VacA against tyrosine=729 and threonine=672, indicating the effectiveness of podophyllotoxin against VacA. Podophyllotoxin against cagA and Urease also showed a variety of hydrogen bonds respectively with lysine, serine, and tyrosine - glutamine, arginine, and asparagine. These results demonstrate the excellent inhibition of podophyllotoxin against Helicobacter pylori. Helicobacter pylori plays a key role in the development of gastric cancer. The transplant conformations predicted in this study showed that podophyllotoxin has valuable inhibitory potential. Therefore, podophyllotoxin may be considered as an anti- Helicobacter pylori agent for further research into drug development.